RAB13 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83336-2-RR (same clone as 83336-2-PBS)

  WB result of RAB13 antibody (83336-2-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00354-1

  Cytometric bead array standard curve of MP00354-1, RAB13 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83336-2-PBS. Detection antibody: 83336-1-PBS. Standard: Ag34898. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83336-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83336-2-RR against Human RAB13 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

RAB13 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83336-2-PBS

Clone No.240216D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

GIG4, 240216D10, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13

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Product Information

83336-2-PBS targets RAB13 as part of a matched antibody pair:

MP00354-1: 83336-2-PBS capture and 83336-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34898

  Product name: Recombinant human RAB13 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-200 aa of BC000799

  Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC

• Full Name: RAB13, member RAS oncogene family
• Calculated Molecular Weight: 203 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000799
• Gene Symbol: RAB13
• Gene ID (NCBI): 5872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51153
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).