Tested Applications
| Positive WB detected in | LNCaP cells, pig brain tissue, HCT 116 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse ovary tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67953-1-Ig targets RAB14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8167 Product name: Recombinant human RAB14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-215 aa of BC006081 Sequence: MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC Predict reactive species |
| Full Name | RAB14, member RAS oncogene family |
| Calculated Molecular Weight | 215 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC006081 |
| Gene Symbol | RAB14 |
| Gene ID (NCBI) | 51552 |
| RRID | AB_2918705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61106 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB14 antibody 67953-1-Ig | Download protocol |
| IHC protocol for RAB14 antibody 67953-1-Ig | Download protocol |
| WB protocol for RAB14 antibody 67953-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB14 Monoclonal antibody (3G7B3), catalog number 67953-1-Ig, has 1 total citation. The citation appears in BMC Cancer, a peer-reviewed journal covering cancer research. The associated research field is gastric cancer, specifically investigating the regulation of natural killer cell infiltration and tumor progression by CMTM6. The antibody was used in Western Blot (WB) with human samples.
| Species | Application | Title |
|---|---|---|
BMC Cancer Regulation of natural killer cell infiltration and tumor progression by CMTM6 in gastric cancer. |
