Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse liver tissue, rat pancreas tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat liver tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:175-1:700 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67659-1-Ig targets RAB18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16669 Product name: Recombinant human RAB18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-206 aa of BC015014 Sequence: MDEDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVKLSHREEGQGGGACGGYCSVL Predict reactive species |
| Full Name | RAB18, member RAS oncogene family |
| Calculated Molecular Weight | 206 aa, 23 kDa |
| GenBank Accession Number | BC015014 |
| Gene Symbol | RAB18 |
| Gene ID (NCBI) | 22931 |
| RRID | AB_2882856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NP72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| IHC protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| WB protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB18 Monoclonal antibody (2E4B3), catalog number 67659-1-Ig, has 1 total citation. The cited publication appeared in J Gastroenterol Hepatol (2024) and investigated the role of Rab18 in liver injury and lipid accumulation by regulating perilipin 2 and PPARγ in non-alcoholic fatty liver disease (NAFLD). The associated research field is hepatology/liver disease, specifically NAFLD pathogenesis. The antibody was used in mouse samples.
| Species | Application | Title |
|---|---|---|
J Gastroenterol Hepatol Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator-activated receptor gamma in non-alcoholic fatty liver disease |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 out of 5 stars. The user performed a Western Blot at a 1:2,000 dilution on HEK293T total cell lysates and confirmed that the antibody effectively detects endogenous Rab18. The reviewer described it as a "very good antibody," indicating reliable performance and strong detection capability for its intended target. Overall, the feedback reflects satisfaction with the product's specificity and sensitivity in standard Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (08-05-2025) | Detects endogenous Rab18 in HEK293T total cell lysates. Very good antibody.
|
