RAB27A-specific Polyclonal antibody

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• KD/KO Validated

RAB27A-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16868-1-AP

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAB27A-specific, RAM, Ras related protein Rab 27A

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Validation Data Gallery

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  WB analysis using 16868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K562 using 16868-1-AP

  WB result of RAB27A antibody (16868-1-AP, 1:1000) with si-Control and si-RAB27A transfected K562 cells.

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  WB analysis of HL-60 using 16868-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, K562, PC-12 cells, U-87 MG cells
• Positive IHC detected in: human lymphoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16868-1-AP targets RAB27A-specific in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB27A, member RAS oncogene family
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_004580
• Gene Symbol: RAB27A
• Gene ID (NCBI): 5873
• RRID: AB_2176716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress; Authors - Lay Thant