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RAB27A-specific Polyclonal antibody

RAB27A-specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16868-1-AP

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A



Tested Applications

Positive WB detected inHL-60 cells, K562 , K-562 cells
Positive IHC detected inhuman lymphoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16868-1-AP targets RAB27A-specific in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_004580
Gene symbol RAB27A
Gene ID (NCBI) 5873
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB27A-specific antibody 16868-1-APDownload protocol
IHC protocol for RAB27A-specific antibody 16868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols