RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E1B10

Cat no : 67501-1-Ig

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A



Tested Applications

Positive WB detected inU2OS cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HeLa cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67501-1-Ig targets RAB2A in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB2A fusion protein Ag7939
Full Name RAB2A, member RAS oncogene family
Calculated Molecular Weight24 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008929
Gene Symbol RAB2A
Gene ID (NCBI) 5862
RRIDAB_2882725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.

Protocols

Product Specific Protocols
WB protocol for RAB2A antibody 67501-1-IgDownload protocol
IHC protocol for RAB2A antibody 67501-1-IgDownload protocol
IF protocol for RAB2A antibody 67501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols