CoraLite®594-conjugated RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E1B10

Cat no : CL594-67501

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67501

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 RAB2A antibody (CL594-67501, Clone: 3E1B10 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67501

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB2A (CL594-67501, Clone:3E1B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse brain tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67501 targets RAB2A in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB2A fusion protein Ag7939
• Full Name: RAB2A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC008929
• Gene Symbol: RAB2A
• Gene ID (NCBI): 5862
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex.