RAB2B Polyclonal antibody

RAB2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11756-1-AP

Synonyms

RAB2B, Ras related protein Rab 2B

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Validation Data Gallery

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  WB analysis of human brain using 11756-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11756-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11756-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11756-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue, mouse kidney tissue, HeLa cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11756-1-AP targets RAB2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB2B fusion protein Ag2352
• Full Name: RAB2B, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC020839
• Gene Symbol: RAB2B
• Gene ID (NCBI): 84932
• RRID: AB_2176900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Publications

Species	Application	Title
human	IHC	Dis Markers; Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma; Authors - Xiubing Chen