RAB39B Monoclonal antibody

RAB39B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4F7

Cat no : 68014-1-Ig

Synonyms

RAB39B, Ras related protein Rab 39B

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Validation Data Gallery

• 

  WB analysis of rabbit brain using 68014-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68014-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 68014-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 68014-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 68014-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68014-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68014-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68014-1-Ig (RAB39B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rabbit brain tissue, Jurkat cells, LNCaP cells, pig brain tissue, mouse brain tissue, pig cerebellum tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68014-1-Ig targets RAB39B in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB39B fusion protein Ag27170
• Full Name: RAB39B, member RAS oncogene family
• Calculated Molecular Weight: 213 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC009714
• Gene Symbol: RAB39B
• Gene ID (NCBI): 116442
• RRID: AB_2918760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB39B is a member of the RAB GTPase family with a putative role in vesicle trafficking. RAB39B is predominantly expressed in neuronal cells and is localized to the endoplasmic reticulum, Golgi and trans‐Golgi recycling endosomes. Several reports suggested that RAB39B may regulate protein trafficking and the PI3K-AKT-mTOR pathway. Mutations in RAB39B are known to be associated with X-linked intellectual disability (XLID), Parkinson's disease, and autism.