Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, U-251 cells, rat brain tissue |
| Positive IHC detected in | rat brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15774-1-AP targets RAB3B/C in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8441 Product name: Recombinant human RAB3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC005035 Sequence: MASVTDGKTGVKDASDQNFDYMFKLLIIGNSSVGKTSFLFRYADDTFTPAFVSTVGIDFKVKTVYRHEKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWATQIKTYSWDNAQVILVGNKCDMEEERVVPTEKGQLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMSDSLDTDPSMLGSSKNTRLSDTPPLLQQNCSC Predict reactive species |
| Full Name | RAB3B, member RAS oncogene family |
| Calculated Molecular Weight | 219 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC005035 |
| Gene Symbol | RAB3B |
| Gene ID (NCBI) | 5865 |
| RRID | AB_2177414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20337 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RAB3B/C antibody 15774-1-AP | Download protocol |
| IF protocol for RAB3B/C antibody 15774-1-AP | Download protocol |
| IHC protocol for RAB3B/C antibody 15774-1-AP | Download protocol |
| WB protocol for RAB3B/C antibody 15774-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RAB3B (Cat# 15774-1-AP) has 11 citations published in journals including Nature Communications, Advanced Science, Cell Death & Disease, Annals of Neurology, Cancer Cell International, Frontiers in Pharmacology, Scientific Reports, Biology of Reproduction, Human Molecular Genetics, and Clinical and Translational Medicine. Research fields span virology, pancreatic islet biology, endothelial cell function, diabetic kidney disease, neuroscience (Huntington's and Alzheimer's disease), oncology (prostate and lung cancer, pan-cancer analysis), and reproductive biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
| ||
Adv Sci (Weinh) Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase | ||
Cell Death Dis Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B | ||
Cancer Cell Int Discovery and validation of RAB3B as a diagnostic biomarker for prostate cancer with serum PSA below 10 ng/mL based on a multi-omics study.
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