RAB3GAP1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66688-1-Ig (same clone as 66688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66688-1-Ig (same clone as 66688-1-PBS)

  WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51694-1

  Cytometric bead array sample test of MP51694-1, RAB3GAP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66688-1-PBS. Detection antibody: 66688-2-PBS.

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  Cytometric bead array standard curve of MP51694-1

  Cytometric bead array standard curve of MP51694-1, RAB3GAP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66688-1-PBS. Detection antibody: 66688-2-PBS. Standard:Ag17710. Range: 0.781-100 ng/mL.

• Featured Product
• KD/KO Validated

RAB3GAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66688-1-PBS

Clone No.4A3A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

4A3A1, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130

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Product Information

66688-1-PBS targets RAB3GAP1 as part of a matched antibody pair:

MP51694-1: 66688-1-PBS capture and 66688-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17710

  Product name: Recombinant human RAB3GAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 502-851 aa of BC146809

  Sequence: PGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIH

• Full Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Calculated Molecular Weight: 981 aa, 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146809
• Gene Symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• RRID: AB_2882042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15042
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.