Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66688-1-Ig targets RAB3GAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17710 Product name: Recombinant human RAB3GAP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 502-851 aa of BC146809 Sequence: PGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIH Predict reactive species |
| Full Name | RAB3 GTPase activating protein subunit 1 (catalytic) |
| Calculated Molecular Weight | 981 aa, 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC146809 |
| Gene Symbol | RAB3GAP1 |
| Gene ID (NCBI) | 22930 |
| RRID | AB_2882042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15042 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| IHC protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| WB protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody rates it 5 stars. The reviewer used it for Western Blot on mouse brain and postmortem brain lysates at a 1:3000 dilution, reporting it as a very good antibody for probing RAB3GAP1, confirming the expected molecular weight of 130 kDa. Overall, the feedback highlights reliable specificity and appropriate band recognition in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
bing (Verified Customer) (08-04-2021) | very good Ab to probe RAB3GAP1 by WB with the right MW of 130 kd
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