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RAB7A Recombinant monoclonal antibody, PBS Only (84741-1-PBS)

RAB7A Recombinant monoclonal antibody, PBS Only

Name: RAB7A Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 84741-1-PBS-100UG

RAB7A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84741-1-PBS

Clone No. 241948B4

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RAB7, 241948B4, EC:3.6.5.2, Ras related protein Rab 7a, Ras-related protein Rab-7a

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 84741-1-RR (same clone as 84741-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsil using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsil using 84741-1-RR (same clone as 84741-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84741-1-RR (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 84741-1-RR (same clone as 84741-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 84741-1-RR (same clone as 84741-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of A431 using 84741-1-RR (same clone as 84741-1-PBS)
1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
FC experiment of K-562 using 84741-1-RR (same clone as 84741-1-PBS)
1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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Tested Applications
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Product Information

84741-1-PBS targets RAB7A in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	RAB7A, member RAS oncogene family
Calculated Molecular Weight	23 kDa
Observed Molecular Weight	23 kDa
GenBank Accession Number	NM_004637
Gene Symbol	RAB7A
Gene ID (NCBI)	7879
RRID	AB_3672126
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P51149
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.