RAB8A Polyclonal antibody, PBS Only

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• RAB8A Polyclonal antibody, PBS Only (55296-1-PBS)

• 

  WB analysis using 55296-1-AP (same clone as 55296-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 55296-1-AP (same clone as 55296-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55296-1-AP (same clone as 55296-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 55296-1-AP (same clone as 55296-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 55296-1-AP (same clone as 55296-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 55296-1-AP (same clone as 55296-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 55296-1-AP (same clone as 55296-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 55296-1-AP (same clone as 55296-1-PBS)

  IP result of anti-RAB8A (IP:55296-1-AP, 4ug; Detection:55296-1-AP 1:600) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55296-1-AP (same clone as 55296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55296-1-AP (same clone as 55296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 55296-1-AP (same clone as 55296-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB8A antibody (55296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55296-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RAB8A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 55296-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

55296-1-PBS targets RAB8A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: RAB8A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_005370
• Gene Symbol: RAB8A
• Gene ID (NCBI): 4218
• RRID: AB_10858398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61006
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.