RABAC1 Polyclonal antibody

RABAC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10542-1-AP

Synonyms

Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1

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Validation Data Gallery

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  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 10542-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HEK-293 using 10542-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10542-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10542-1-AP targets RABAC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABAC1 fusion protein Ag0830
• Full Name: Rab acceptor 1 (prenylated)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC008950
• Gene Symbol: RABAC1
• Gene ID (NCBI): 10567
• RRID: AB_2175639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	BMC Neurosci; A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.; Authors - Dickison Virginia M VM
mouse,human	WB,IF	PLoS One; Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.; Authors - Ameair Abu Irqeba
mouse	WB	Biochim Biophys Acta Proteins Proteom; Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.; Authors - Uchenna Emechebe