Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human endometrial cancer tissue, rat liver tissue, mouse liver tissue, rat testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12735-1-AP targets RabGEF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3442 Product name: Recombinant human RABGEF1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 136-491 aa of BC015330 Sequence: DRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG Predict reactive species |
| Full Name | RAB guanine nucleotide exchange factor (GEF) 1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC015330 |
| Gene Symbol | RABGEF1 |
| Gene ID (NCBI) | 27342 |
| RRID | AB_2175788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJ41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RabGEF1 (Rabex-5) is a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator Rab5. RabGEF1 contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain (PMID: 17341663). RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It is also implicated in tumorigenesis and in the development of certain human cancers (PMID: 25427001).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| IHC protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| WB protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RabGEF1 Polyclonal antibody (Cat# 12735-1-AP) has 9 total citations. These appear in journals including J Clin Invest, ACS Cent Sci, Cell Reports, Frontiers in Cell and Developmental Biology, Scientific Reports, FASEB Journal, Experimental Molecular Pathology, Journal of Cell Science, and Orthopaedic Surgery. Research fields span lung adenocarcinoma, DNA replication stress, Alzheimer's disease, endosomal trafficking, phagolysosome biology, glioblastoma, and osteoarthritis, with applications primarily in Western blot and immunofluorescence across human, mouse, rat, and zebrafish.
| Species | Application | Title |
|---|---|---|
J Clin Invest Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progression. | ||
ACS Cent Sci Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1 | ||
Cell Rep Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. | ||
Sci Rep AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. | ||
FASEB J ATP7A Maintains Bactericidal Function of Neutrophils and Macrophages via Regulating the Formation and Activation of Phagolysosomes |
Reviews
What customers say
The single review for 12735-1-AP is a 5-star rating. The customer used the antibody for Western Blot at a 1:500 dilution on hippocampus homogenate and reported that it "worked well." Overall, the feedback is positive, indicating reliable performance in the tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kuldeep (Verified Customer) (05-27-2025) | Worked well.
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