RACK1; GNB2L1 Monoclonal antibody

RACK1; GNB2L1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E11A7

Cat no : 66940-1-Ig

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1



Tested Applications

Positive WB detected inHeLa cells, pig liver tissue, NIH/3T3 cells, HSC-T6 cells, HepG2 cells, T-47D cells, L02 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66940-1-Ig targets RACK1; GNB2L1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RACK1; GNB2L1 fusion protein Ag26708
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC019093
Gene symbol GNB2L1
Gene ID (NCBI) 10399
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Protocols

Product Specific Protocols
WB protocol for RACK1; GNB2L1 antibody 66940-1-IgDownload protocol
IHC protocol for RACK1; GNB2L1 antibody 66940-1-IgDownload protocol
IF protocol for RACK1; GNB2L1 antibody 66940-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols