CoraLite®594-conjugated RACK1; GNB2L1 Monoclonal antibody

RACK1; GNB2L1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

1E11A7

Cat no : CL594-66940

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66940

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 RACK1; GNB2L1 antibody (CL594-66940, Clone: 1E11A7 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IF: See 1 publications below

Product Information

CL594-66940 targets RACK1; GNB2L1 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC019093
• Gene Symbol: GNB2L1
• Gene ID (NCBI): 10399
• RRID: AB_2920060
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publications

Species	Application	Title
mouse	IF	Vet Res; RACK1 mediates NLRP3 inflammasome activation during Pasteurella multocida infection; Authors - Jinrong Ran ; KD Validated