Tested Applications
| Positive WB detected in | C6 cells, Jurkat cells, K-562 cells, MCF-7 cells, MOLT-4 cells |
| Positive IHC detected in | human breast cancer tissue, mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | U2OS cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
29390-1-AP targets RAD50 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29949 Product name: Recombinant human RAD50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 275-425 aa of NM_005732 Sequence: LDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLK Predict reactive species |
| Full Name | RAD50 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 154 kDa |
| Observed Molecular Weight | 154 kDa |
| GenBank Accession Number | NM_005732 |
| Gene Symbol | RAD50 |
| Gene ID (NCBI) | 10111 |
| RRID | AB_2918289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92878 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAD50 antibody 29390-1-AP | Download protocol |
| IHC protocol for RAD50 antibody 29390-1-AP | Download protocol |
| WB protocol for RAD50 antibody 29390-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis | ||
Ecotoxicol Environ Saf Adolescent cetylpyridinium chloride exposure impairs homologous recombination repair and induces granulosa cell apoptosis and follicular atresia via FOXM1/CREBBP complex suppression | ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors | ||
CNS Neurosci Ther Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Arnaud (Verified Customer) (09-26-2025) | Work very well at 1:2000 dilution
|
FH Veronica (Verified Customer) (11-14-2022) | The antibody seems to work correctly. However, further tests need to be done.
|
