CoraLite® Plus 488-conjugated RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29390

Synonyms

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

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Validation Data Gallery

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  IF Staining of U2OS using CL488-29390

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 RAD50 antibody (CL488-29390) at dilution of 1:200.

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  FC experiment of HeLa using CL488-29390

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD50 (CL488-29390) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: U2OS cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-29390 targets RAD50 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD50 fusion protein Ag29949
• Full Name: RAD50 homolog (S. cerevisiae)
• Calculated Molecular Weight: 154 kDa
• Observed Molecular Weight: 154 kDa
• GenBank Accession Number: NM_005732
• Gene Symbol: RAD50
• Gene ID (NCBI): 10111
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.