RAF1 Polyclonal antibody

RAF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26863-1-AP

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1



Tested Applications

Positive WB detected inDU 145 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26863-1-AP is RAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAF1 fusion protein Ag25423
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated molecular weight 648 aa, 73 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC018119
Gene symbol RAF1
Gene ID (NCBI) 5894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Protocols

Product Specific Protocols
WB protocol for RAF1 antibody 26863-1-APDownload protocol
IHC protocol for RAF1 antibody 26863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.

Authors - Dali Zhao
humanWB

Oncogene

Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.

Authors - Feifei Zhang