RAF1 Polyclonal antibody

RAF1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 51140-1-AP

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

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Validation Data Gallery

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  IHC staining of human prostate cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51140-1-AP targets RAF1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• RRID: AB_2881251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Publications

Species	Application	Title
human		Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse		J Neuroinflammation; Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.; Authors - Xiaowei Fei
human		Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human		Biochem Pharmacol; 1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.; Authors - Yuhang Zhou
mouse		Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human		Food Funct; Dandelion root extract affects ESCC progression via regulating multiple signal pathways.; Authors - Xiaofang Duan