RAF1 Polyclonal antibody

RAF1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 51140-1-AP

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1



Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 12 publications below

Product Information

51140-1-AP targets RAF1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated molecular weight 648 aa, 73 kDa
GenBank accession numberBC018119
Gene symbol RAF1
Gene ID (NCBI) 5894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Protocols

Product Specific Protocols
IHC protocol for RAF1 antibody 51140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Identification of RE1-Silencing Transcription Factor as a Promoter of Metastasis in Pancreatic Cancer.

Authors - Haoyi Jin
humanWB

Oncol Rep

EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.

Authors - Chuan Lin
mouseWB

Biochim Biophys Acta Mol Basis Dis

PMID: 30389579

Authors - Chunling Yuan
humanWB

Biomed Res Int

Inhibition of Specificity Protein 1 Is Involved in Phloretin-Induced Suppression of Prostate Cancer.

Authors - Dan Kang
sheepWB

BMC Microbiol

Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway.

Authors - Yanan Li
humanWB

Biochem Biophys Res Commun

Sex hormone-binding globulin regulates the activity of the ERK pathway in the placentas of patients with gestational diabetes mellitus.

Authors - Xiaoyan Wang