RAG2 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• RAG2 Polyclonal antibody, PBS Only (11825-1-PBS)

• 

  WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  IP result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:500) with mouse thymus tissue lysate 1000 ug. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11825-1-AP (same clone as 11825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11825-1-AP (same clone as 11825-1-PBS)

  Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

RAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

RAG 2, RAG-2, V(D)J recombination-activating protein 2

---

Product Information

11825-1-PBS targets RAG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2393

  Product name: Recombinant human RAG2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 175-527 aa of BC022397

  Sequence: VADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNTGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

• Full Name: recombination activating gene 2
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 57-62 kDa
• GenBank Accession Number: BC022397
• Gene Symbol: RAG2
• Gene ID (NCBI): 5897
• RRID: AB_2238062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55895
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3