RALY Monoclonal antibody, PBS Only

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  WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68011-1-Ig (RALY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RALY antibody (68011-1-Ig, Clone: 2B10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RALY (68011-1-Ig, Clone:2B10C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

RALY Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68011-1-PBS

Clone No.2B10C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

2B10C3, Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2

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Product Information

68011-1-PBS targets RALY in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12250

  Product name: Recombinant human RALY protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-307 aa of BC103753

  Sequence: MSLKLQASNVTNKNDPKSINSRVFIGNLNTALVKKSDVETIFSKYGRVAGCSVHKGYAFVQYSNERHARAAVLGENGRVLAGQTLDINMAGEPKPDRPKGLKRAASAIYSGYIFDYDYYRDDFYDRLFDYRGRLSPVPVPRAVPVKRPRVTVPLVRRVKTNVPVKLFARSTAVTTSSAKIKLKSSELQAIKTELTQIKSNIDALLSRLEQIAAEQKANPDGKKKGDGGGASGGGGGGGGSGGGGSGGGGGGGSSRPPAPQENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDGALQ

• Full Name: RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
• Calculated Molecular Weight: 307 aa, 33 kDa
• Observed Molecular Weight: 30-37 kDa
• GenBank Accession Number: BC103753
• Gene Symbol: RALY
• Gene ID (NCBI): 22913
• RRID: AB_2918758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UKM9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. RALYhas two isoforms with the molecular weight of 30 kDa and 32 kDa, and it has many modification sites, which made it shows at 30-37 kDa by Western Blot.