RAMP1 Polyclonal antibody

RAMP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10327-1-AP

Synonyms

RAMP1

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Validation Data Gallery

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  WB analysis using 10327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10327-1-AP (RAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10327-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10327-1-AP (RAMP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10327-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10327-1-AP (RAMP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10327-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10327-1-AP (RAMP1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10327-1-AP targets RAMP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAMP1 fusion protein Ag0402
• Full Name: receptor (G protein-coupled) activity modifying protein 1
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 14 kDa, 35 kDa
• GenBank Accession Number: BC000548
• Gene Symbol: RAMP1
• Gene ID (NCBI): 10267
• RRID: AB_2269270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAMP1 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region, and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. RAMP1 can form a homodimer which migrates at 30-37 kDa on SDS-PAGE. (PMID: 9620797; 16188935; 12051717;PMID: 18384073)

Publications

Species	Application	Title
rat	IF	J Headache Pain; Calcitonin gene-related peptide receptor antagonist BIBN4096BS regulates synaptic transmission in the vestibular nucleus and improves vestibular function via PKC/ERK/CREB pathway in an experimental chronic migraine rat model.; Authors - Ruimin Tian
rat	WB,IHC	Front Pharmacol; Effect of Xiongmatang Extract on Behavioral and TRPV1-CGRP/CGRP-R Pathway in Rats With Migraine.; Authors - Dingding Liu
rat	IF	Neurosci Lett; Calcitonin gene-related peptide differentially regulates gene and protein expression in trigeminal glia cells: findings from array analysis.; Authors - Vause Carrie V CV