RAMP2 Polyclonal antibody

RAMP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13223-2-AP

Synonyms

RAMP2

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Validation Data Gallery

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  WB analysis of mouse brain using 13223-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13223-2-AP (RAMP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13223-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13223-2-AP (RAMP2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13223-2-AP targets RAMP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAMP2 fusion protein Ag4028
• Full Name: receptor (G protein-coupled) activity modifying protein 2
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC027975
• Gene Symbol: RAMP2
• Gene ID (NCBI): 10266
• RRID: AB_2176337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAMP2 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. (PMID: 9620797; 16188935)

Publications

Species	Application	Title
human, mouse	WB	FASEB J; Anti-inflammatory actions of adrenomedullin through fine tuning of HIF stabilization.; Authors - MacManus Christopher F CF
bovine	IHC	Reprod Biol Endocrinol; Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta.; Authors - Hayashi Ken-Go KG
mouse,human	WB	FEBS Open Bio; Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin-4 expression in the colonic epithelium; Authors - Makiko Kawaguchi