RAP1GAP Recombinant antibody

RAP1GAP Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 86011-1-RR
Clone No.250422B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inA549 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86011-1-RR targets RAP1GAP in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAP1GAP fusion protein Ag5576 Predict reactive species
Full Name RAP1 GTPase activating protein
Calculated Molecular Weight 73 kDa
GenBank Accession NumberBC054490
Gene Symbol RAP1GAP
Gene ID (NCBI) 5909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).

Protocols

Product Specific Protocols
IF protocol for RAP1GAP antibody 86011-1-RRDownload protocol
Standard Protocols
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