RAP1GAP Recombinant monoclonal antibody

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  IF Staining of A549 using 86011-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAP1GAP antibody (86011-1-RR, Clone: 250422B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Jurkat using 86011-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAP1GAP antibody (86011-1-RR, Clone: 250422B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RAP1GAP Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 86011-1-RR

Clone No.250422B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

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Tested Applications

• Positive IF/ICC detected in: A549 cells, Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86011-1-RR targets RAP1GAP in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5576

  Product name: Recombinant human RAP1GAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 313-663 aa of BC054490

  Sequence: FLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC

• Full Name: RAP1 GTPase activating protein
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: BC054490
• Gene Symbol: RAP1GAP
• Gene ID (NCBI): 5909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).