RAPGEFL1 Polyclonal antibody

• 

  WB analysis of human brain using 21746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21746-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21746-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAPGEFL1 antibody 21746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

RAPGEFL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 21746-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Link GEFII, RAPGEFL1

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21746-1-AP targets RAPGEFL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16506

  Product name: Recombinant human RAPGEFL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-290 aa of BC115374

  Sequence: MEGPEGLGRKQACLAMLLHFLDTYQGLLQEEEGAGHIIKDLYLLIMKDESLYQGLREDTLRLHQLVETVELKIPEENQPPSKQVKPLFRHFRRIDSCLQTRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVQDILGSVTEKLQYSEEPAGREDSLILVAVSSSGEKVLLQPTEDCVFTALGINSHLFACTRDSYEALVPLPEEIQVSPGDTEIHRVEPEDVANHLTAFHWELFRCVHELEFVDYVFHGERGRRETANLELLLQRCSEVTHWVATEVLLCEAPGKRAQLL

• Full Name: Rap guanine nucleotide exchange factor (GEF)-like 1
• Calculated Molecular Weight: 662 aa, 73 kDa
• Observed Molecular Weight: 73 kDa, 59 kDa
• GenBank Accession Number: BC115374
• Gene Symbol: RAPGEFL1
• Gene ID (NCBI): 51195
• RRID: AB_10733650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHV5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.