RARA Polyclonal antibody

RARA Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10331-1-AP

Synonyms

retinoic acid receptor, alpha, Retinoic acid receptor alpha, RAR-alpha, RAR alpha, RAR

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Validation Data Gallery

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  WB analysis of K-562 using 10331-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10331-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10331-1-AP

  IP result of anti-RARA (IP:10331-1-AP, 4ug; Detection:10331-1-AP 1:2000) with HeLa cells lysate 1760 ug.

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human testis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

10331-1-AP targets RARA in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RARA fusion protein Ag0420
• Full Name: retinoic acid receptor, alpha
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC008727
• Gene Symbol: RARA
• Gene ID (NCBI): 5914
• RRID: AB_2177742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoic acid receptors (RARs) function as nuclear receptors that control gene expression in response to binding of the ligand retinoic acid (RA). Retinoic acid receptor alpha (RARα), also known as NR1B1 (nuclear receptor subfamily 1, group B, member 1), plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758).

Publications

Species	Application	Title
human	WB	Br J Cancer; All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex; Authors - Lu Wang
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB	Front Cell Dev Biol; Silybin Prevents Prostate Cancer by Inhibited the ALDH1A1 Expression in the Retinol Metabolism Pathway.; Authors - Ying Jiang
human	IF	Stem Cell Rev Rep; Pulse Activation of Retinoic Acid Receptor Enhances Hematopoietic Stem Cell Homing by Controlling CXCR4 Membrane Presentation; Authors - Nanxi Geng
human	WB	Cell Signal; ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury.; Authors - Zhen Yu
human	IF	Andrology; A novel SNP in HUWE1 promoter confers increased risk of NOA by affecting the RA/RARα pathway in Chinese individuals; Authors - Xudong Shan