Tested Applications
| Positive WB detected in | mouse testis tissue, mouse heart tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11569-1-AP targets RASEF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2149 Product name: Recombinant human RASEF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 354-740 aa of BC023566 Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG Predict reactive species |
| Full Name | RAS and EF-hand domain containing |
| Calculated Molecular Weight | 740 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC023566 |
| Gene Symbol | RASEF |
| Gene ID (NCBI) | 158158 |
| RRID | AB_2238112 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZ41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RASEF belongs to the Rab family of small GTPases, which function as molecular switches cycling between GDP-bound (inactive) and GTP-bound (active) states. RASEF is predicted to be involved in regulating membrane traffic and vesicle-mediated transport. Its ability to interact with dynein and dynactin components suggests a specific role in microtubule-based transport processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RASEF antibody 11569-1-AP | Download protocol |
| IP protocol for RASEF antibody 11569-1-AP | Download protocol |
| WB protocol for RASEF antibody 11569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RASEF Polyclonal antibody (Cat# 11569-1-AP) has 2 citations. They appear in Molecular Cancer Research (2013) and International Journal of Clinical and Experimental Pathology (2018). The research fields are oncology: one study identifies RASEF as a diagnostic biomarker and therapeutic target for lung cancer, while the other associates high RASEF expression with better prognosis in colorectal cancer. Applications cited include WB, IP, IHC, and IF in human samples.
| Species | Application | Title |
|---|---|---|
Mol Cancer Res RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.
| ||
Int J Clin Exp Pathol High RASEF expression is associated with a significantly better prognosis in colorectal cancer |
