RASEF Polyclonal antibody

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  WB analysis of mouse testis using 11569-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11569-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11569-1-AP

  IP result of anti-RASEF (IP:11569-1-AP, 4ug; Detection:11569-1-AP 1:1500) with mouse testis tissue lysate 1520 ug.

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  IHC staining of mouse brain using 11569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RASEF Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11569-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse heart tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11569-1-AP targets RASEF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2149

  Product name: Recombinant human RASEF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 354-740 aa of BC023566

  Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

• Full Name: RAS and EF-hand domain containing
• Calculated Molecular Weight: 740 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC023566
• Gene Symbol: RASEF
• Gene ID (NCBI): 158158
• RRID: AB_2238112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZ41
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASEF belongs to the Rab family of small GTPases, which function as molecular switches cycling between GDP-bound (inactive) and GTP-bound (active) states. RASEF is predicted to be involved in regulating membrane traffic and vesicle-mediated transport. Its ability to interact with dynein and dynactin components suggests a specific role in microtubule-based transport processes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IHC,IF	Mol Cancer Res; RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.; Authors - Oshita Hideto H ; KD Validated
human	IHC	Int J Clin Exp Pathol; High RASEF expression is associated with a significantly better prognosis in colorectal cancer; Authors - Xin Yu