RASEF Monoclonal antibody, PBS Only

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  WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67159-1-PBS

Clone No.1C2B2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

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Product Information

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2149

  Product name: Recombinant human RASEF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 354-740 aa of BC023566

  Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

• Full Name: RAS and EF-hand domain containing
• Calculated Molecular Weight: 740 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC023566
• Gene Symbol: RASEF
• Gene ID (NCBI): 158158
• RRID: AB_2882456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IZ41
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.