Filter: AllWBIHCIF

WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

RASEF Monoclonal antibody, PBS Only

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67159-1-PBS

Clone No.1C2B2

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity	Human, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	RASEF fusion protein Ag2149 Predict reactive species
Full Name	RAS and EF-hand domain containing
Calculated Molecular Weight	740 aa, 83 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC023566
Gene Symbol	RASEF
Gene ID (NCBI)	158158
RRID	AB_2882456
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8IZ41
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.