RASEF Monoclonal antibody

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2B2

Cat no : 67159-1-Ig

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

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Validation Data Gallery

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  WB analysis of LNCaP using 67159-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67159-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67159-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: human prostate cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67159-1-Ig targets RASEF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RASEF fusion protein Ag2149
• Full Name: RAS and EF-hand domain containing
• Calculated Molecular Weight: 740 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC023566
• Gene Symbol: RASEF
• Gene ID (NCBI): 158158
• RRID: AB_2882456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.