RASSF6 Polyclonal antibody

• Featured Product
• KD/KO Validated

RASSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11921-1-AP

Synonyms

Ras association domain-containing protein 6

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Validation Data Gallery

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  WB analysis of HT-29 using 11921-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 11921-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11921-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11921-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11921-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11921-1-AP

  IP result of anti-RASSF6 (IP:11921-1-AP, 4ug; Detection:11921-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human ovary tumor using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11921-1-AP

  Immunofluorescent analysis of Hela cells, using RASSF6 antibody 11921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HT-29 cells, COLO 320 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, PC-3 cells, U-937 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

11921-1-AP targets RASSF6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASSF6 fusion protein Ag2565
• Full Name: Ras association (RalGDS/AF-6) domain family member 6
• Calculated Molecular Weight: 369 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC058835
• Gene Symbol: RASSF6
• Gene ID (NCBI): 166824
• RRID: AB_874225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Oncotarget; RASSF6 downregulation promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal cancer.; Authors - Rui Zhou
human	WB	Mol Cancer; The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias.; Authors - Hesson Luke B LB
		Epigenetics; RASSF6 exhibits promoter hypermethylation in metastatic melanoma and inhibits invasion in melanoma cells.; Authors - Jessica Mezzanotte
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng ; KD Validated
human	WB	J Cell Physiol; MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer.; Authors - Hua Wang
human	WB,IHC	Ann Surg Oncol; Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery.; Authors - Wen Yugang Y