RASSF6 Polyclonal antibody

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• RASSF6 Polyclonal antibody (11921-1-AP)

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• KD/KO Validated

RASSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11921-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ras association domain-containing protein 6

• 

  WB analysis of HT-29 using 11921-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 11921-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 11921-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 11921-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 11921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MDA-MB-453s using 11921-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 11921-1-AP

  IP result of anti-RASSF6 (IP:11921-1-AP, 4ug; Detection:11921-1-AP 1:500) with HepG2 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11921-1-AP

  Immunofluorescent analysis of Hela cells, using RASSF6 antibody 11921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-29 cells, COLO 320 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, PC-3 cells, U-937 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11921-1-AP targets RASSF6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2565

  Product name: Recombinant human RASSF6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC058835

  Sequence: MAHQYPSWIFINEKTFITREQLNSLLKTYNIFYENQKNLHILYGETEDGKLIVEGMLDIFWGVKRPIQLKIQDEKPFSSFTSMKSSDVFSSKGMTRWGEFDDLYRISELDRTQIPMSEKRNSQEDYLPYHSNTLKPHAKDEPDSPVLYRTMSEAALVRKRMKPLMMDRKERQKNRASINGHFYNHEIENSPQDFALHIIFATGEQRRLKKTDIPLLQRLLQGPSEKNARIFLMDKDAEEISSDVAQYINFHFSLLESILQRLNEEEKREIQRIVTKFNKEKAIILKCLQNKLVIKTETTV

• Full Name: Ras association (RalGDS/AF-6) domain family member 6
• Calculated Molecular Weight: 369 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC058835
• Gene Symbol: RASSF6
• Gene ID (NCBI): 166824
• RRID: AB_874225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZTQ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RASSF6 antibody (Cat# 11921-1-AP) has 14 citations published in journals including Molecular Cancer, Oncotarget, Journal of Genetics and Genomics, World Journal of Gastroenterology, Journal of Cellular Physiology, Annals of Surgical Oncology, Epigenetics, Molecular and Cellular Biology, Biochemical and Biophysical Research Communications, Onco Targets and Therapy, and Genes Cells. Research fields span tumor suppression, epigenetic inactivation, epithelial-mesenchymal transition, apoptosis, cell cycle regulation, Hippo signaling, p53 pathway, and NF-κB signaling across leukemia, colorectal, gastric, pancreatic, breast, melanoma, esophageal, and bladder cancers.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cancer; The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias.; Authors - Hesson Luke B LB
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng ; KD Validated
human	WB, IHC	World J Gastroenterol; Low RASSF6 expression in pancreatic ductal adenocarcinoma is associated with poor survival.; Authors - Hui-Lin Ye
human,mouse	WB,IHC	Oncotarget; RASSF6 downregulation promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal cancer.; Authors - Rui Zhou
human	WB	J Cell Physiol; MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer.; Authors - Hua Wang
human	WB,IHC	Ann Surg Oncol; Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery.; Authors - Wen Yugang Y