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RASSF6 Polyclonal antibody, PBS Only (11921-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HT-29 using 11921-1-AP (same clone as 11921-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 11921-1-AP (same clone as 11921-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 11921-1-AP (same clone as 11921-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 11921-1-AP (same clone as 11921-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of U-937 using 11921-1-AP (same clone as 11921-1-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 11921-1-AP (same clone as 11921-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11921-1-AP (same clone as 11921-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 11921-1-AP (same clone as 11921-1-PBS)
IP result of anti-RASSF6 (IP:11921-1-AP, 4ug; Detection:11921-1-AP 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 11921-1-AP (same clone as 11921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11921-1-AP (same clone as 11921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11921-1-AP (same clone as 11921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11921-1-AP (same clone as 11921-1-PBS)
Immunofluorescent analysis of Hela cells, using RASSF6 antibody 11921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11921-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RASSF6 Polyclonal antibody, PBS Only

Name: RASSF6 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11921-1-PBS

RASSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11921-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11921-1-PBS targets RASSF6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2565 Product name: Recombinant human RASSF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC058835 Sequence: MAHQYPSWIFINEKTFITREQLNSLLKTYNIFYENQKNLHILYGETEDGKLIVEGMLDIFWGVKRPIQLKIQDEKPFSSFTSMKSSDVFSSKGMTRWGEFDDLYRISELDRTQIPMSEKRNSQEDYLPYHSNTLKPHAKDEPDSPVLYRTMSEAALVRKRMKPLMMDRKERQKNRASINGHFYNHEIENSPQDFALHIIFATGEQRRLKKTDIPLLQRLLQGPSEKNARIFLMDKDAEEISSDVAQYINFHFSLLESILQRLNEEEKREIQRIVTKFNKEKAIILKCLQNKLVIKTETTV Predict reactive species
Full Name	Ras association (RalGDS/AF-6) domain family member 6
Calculated Molecular Weight	369 aa, 43 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	BC058835
Gene Symbol	RASSF6
Gene ID (NCBI)	166824
RRID	AB_874225
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZTQ3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.