Tested Applications
| Positive WB detected in | K-562 cells, HT-1080 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30373-1-AP targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | retinoblastoma 1 |
| Calculated Molecular Weight | 928 aa, 106 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC039060 |
| Gene Symbol | RB1 |
| Gene ID (NCBI) | 5925 |
| RRID | AB_3086301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RB1 antibody 30373-1-AP | Download protocol |
| IHC protocol for RB1 antibody 30373-1-AP | Download protocol |
| WB protocol for RB1 antibody 30373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RB1 Polyclonal antibody (Cat# 30373-1-AP) has 1 total citation. It appears in the journal Clinical and Experimental Medicine, in a study investigating how ALKBH2 promotes the Warburg effect and bladder cancer progression under hypoxic conditions via the PI3K/AKT pathway. The associated research fields include cancer metabolism, tumor progression, and intracellular signaling pathways.
| Species | Application | Title |
|---|---|---|
Clin Exp Med ALKBH2 promotes the Warburg effect and bladder cancer progression under hypoxic conditions via the PI3K/AKT pathway. |
