|Positive WB detected in||HeLa cells, Jurkat cells, mouse brain tissue, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10043-2-AP targets RB1CC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||RB1-inducible coiled-coil 1|
|Calculated molecular weight||1594 aa, 183 kDa|
|Observed molecular weight||180-200 kDa|
|GenBank accession number||BC017556|
|Gene ID (NCBI)||9821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.
The Cargo Receptor NDP52 Initiates Selective Autophagy by Recruiting the ULK Complex to Cytosol-Invading Bacteria.
Biomed Res Int
Repairing and Analgesic Effects of Umbilical Cord Mesenchymal Stem Cell Transplantation in Mice with Spinal Cord Injury.
Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity.
The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-β signaling.
Breast Cancer Res Treat
Autophagy inhibition re-sensitizes pulse stimulation-selected paclitaxel-resistant triple negative breast cancer cells to chemotherapy-induced apoptosis.
J Biol Chem
ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy.