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RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10069-1-AP

Synonyms

CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC



Tested Applications

Positive WB detected inMCF7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 10069-1-AP is RB1CC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag0003
Full Name RB1-inducible coiled-coil 1
Calculated molecular weight 1594 aa, 183 kDa
Observed molecular weight 183 kDa
GenBank accession numberBC017556
Gene symbol RB1CC1
Gene ID (NCBI) 9821
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 10069-1-APDownload protocol
IHC protocol for RB1CC1 antibody 10069-1-APDownload protocol
IF protocol for RB1CC1 antibody 10069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Dev Cell

The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.

Authors - Claudia Puri