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RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, co-IP, ELISA

Conjugate

Unconjugated

Cat no : 17250-1-AP

Synonyms

CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17250-1-AP is RB1CC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag10508
Full Name RB1-inducible coiled-coil 1
Calculated molecular weight 1594 aa, 183 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC017556
Gene symbol RB1CC1
Gene ID (NCBI) 9821
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 17250-1-APDownload protocol
IHC protocol for RB1CC1 antibody 17250-1-APDownload protocol
IF protocol for RB1CC1 antibody 17250-1-APDownload protocol
IP protocol for RB1CC1 antibody 17250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Dev Cell

Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation.

Authors - Suresh Kumar
humanWB,IF

Mol Cell

Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.

Authors - Jose Norberto S Vargas
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang
humanWB

Mol Cell Biol

Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions.

Authors - Motoki Fujimaki
mouseIF

Mol Cell

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Authors - Haruka Chino
mouseIF

Mol Cell Biol

Hyperosmotic stress induces unconventional autophagy independent of the Ulk1 complex.

Authors - Naoki Tamura

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human endometrial stromal cells