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RB1CC1 Polyclonal antibody
RB1CC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17250-1-AP
|Positive WB detected in||HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17250-1-AP is RB1CC1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RB1CC1 fusion protein Ag10508|
|Full Name||RB1-inducible coiled-coil 1|
|Calculated molecular weight||1594 aa, 183 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||BC017556|
|Gene ID (NCBI)||9821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.
|Product Specific Protocols|
|WB protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IHC protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IF protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IP protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SANGAPPA (Verified Customer) (01-04-2020)
Works very good in western blotting!