RBBP9 Monoclonal antibody, PBS Only

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  WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66015-2-Ig (same clone as 66015-2-PBS)

  WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66015-2-Ig (same clone as 66015-2-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RBBP9 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 66015-2-PBS

Clone No.3D5E11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

3D5E11, B5T-overexpressed gene protein, BOG, EC:3.-.-.-, Glutathione S-Transferase

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Product Information

66015-2-PBS targets RBBP9 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17864

  Product name: Recombinant human RBBP9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-186 aa of BC015938

  Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA

• Full Name: retinoblastoma binding protein 9
• Calculated Molecular Weight: 186 aa, 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC015938
• Gene Symbol: RBBP9
• Gene ID (NCBI): 10741
• RRID: AB_11044197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75884
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)