RBM15B Polyclonal antibody

RBM15B Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22249-1-AP

Synonyms

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22249-1-AP is RBM15B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM15B fusion protein Ag17744
Full Name RNA binding motif protein 15B
Calculated molecular weight 890 aa, 97 kDa
Observed molecular weight 100-120 kDa, 70 kDa
GenBank accession numberBC001367
Gene symbol RBM15B
Gene ID (NCBI) 29890
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)

Protocols

Product Specific Protocols
WB protocol for RBM15B antibody 22249-1-APDownload protocol
IP protocol for RBM15B antibody 22249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
humanWB

eNeuro

Altered Expression of the m6A Methyltransferase METTL3 in Alzheimer's Disease.

Authors - He Huang
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated