RBM16 Polyclonal antibody, PBS Only

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• RBM16 Polyclonal antibody, PBS Only (18893-1-PBS)

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  WB analysis of HT-1080 using 18893-1-AP (same clone as 18893-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis using 18893-1-AP (same clone as 18893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 18893-1-AP (same clone as 18893-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 18893-1-AP (same clone as 18893-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 18893-1-AP (same clone as 18893-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18893-1-AP (same clone as 18893-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18893-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

18893-1-PBS targets RBM16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13499

  Product name: Recombinant human RBM16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 921-1271 aa of BC070071

  Sequence: MPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT

• Full Name: RNA binding motif protein 16
• Calculated Molecular Weight: 1271 aa, 141 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC070071
• Gene Symbol: RBM16
• Gene ID (NCBI): 22828
• RRID: AB_2175908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPN6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )