RBM19 Polyclonal antibody, PBS Only

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• RBM19 Polyclonal antibody, PBS Only (19095-1-PBS)

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  WB analysis of mouse colon using 19095-1-AP (same clone as 19095-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 19095-1-AP (same clone as 19095-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 19095-1-AP (same clone as 19095-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19095-1-AP (same clone as 19095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 19095-1-AP (same clone as 19095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 19095-1-AP (same clone as 19095-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

RBM19 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19095-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19095-1-PBS targets RBM19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13545

  Product name: Recombinant human RBM19 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC006137

  Sequence: MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQKHFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKVAGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQESEEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGSEAEEEDSSATPVLQERDSRGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRG

• Full Name: RNA binding motif protein 19
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC006137
• Gene Symbol: RBM19
• Gene ID (NCBI): 9904
• RRID: AB_10640906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4C8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.