RBM24 Recombinant monoclonal antibody

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  WB analysis using 85674-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85674-1-RR (RBM24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RBM24 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85674-1-RR

Clone No.243006A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

RNA binding motif protein 24, RNA binding protein 24, RNA-binding motif protein 24, RNA-binding protein 24, RNA-binding region-containing protein 6

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85674-1-RR targets RBM24 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12871

  Product name: Recombinant human RBM24 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-191 aa of BC104808

  Sequence: MYVCLCVSVAKVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRIMQPGFAFGVQQLHPALIQRPFGIPAHYVYPQAFVQPGVVIPHVQPTAAAASTTPYIDYTGAAYAQYSAAAAAAAAAAAYDQYPYAASPAAAGYVTAGGYGYAVQQPITAAAPGTAAAAAAAAAAAAAFGQYQPQQLQTDRMQ

• Full Name: RNA binding motif protein 24
• Calculated Molecular Weight: 191 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC104808
• Gene Symbol: RBM24
• Gene ID (NCBI): 221662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BX46
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).