RBM25 Polyclonal antibody

RBM25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25297-1-AP

Synonyms

fSAP94, NET52, Protein S164, RBM25, RED120, RNA binding motif protein 25, RNA binding protein 25, RNPC7, S164

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Validation Data Gallery

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  WB analysis using 25297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25297-1-AP (RBM25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25297-1-AP

  IP result of anti-RBM25 (IP:25297-1-AP, 4ug; Detection:25297-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human cervical cancer using 25297-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 25297-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25297-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (25297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Product Information

25297-1-AP targets RBM25 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM25 fusion protein Ag18244
• Full Name: RNA binding motif protein 25
• Calculated Molecular Weight: 843 aa, 100 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC113389
• Gene Symbol: RBM25
• Gene ID (NCBI): 58517
• RRID: AB_2880014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

Publications

Species	Application	Title
mouse	RIP	Arterioscler Thromb Vasc Biol; Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice; Authors - Cui Ma
rat	IP	PeerJ; RBM25 binds to and regulates alternative splicing levels of Slc38a9, Csf1, and Coro6 to affect immune and inflammatory processes in H9c2 cells; Authors - Xin Tian
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
mouse	WB	Gene; ZC3H13 may participate in the ferroptosis process of sepsis-induced cardiomyopathy by regulating the expression of Pnn and Rbm25; Authors - Wenji Lin
human	WB,IHC	Sci China Life Sci; RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2; Authors - Lili Zhi ; KD Validated