Multi-rAb® RBM25 Multi-Recombinant antibody

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  WB analysis using RMX00066

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using RMX00066

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using RMX00066

  IP result of anti-RBM25 (IP:RMX00066, 4ug; Detection:RMX00066 1:5000) with HEK-293 cells lysate 1000 ug.

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  IHC staining of human colon cancer using RMX00066

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using RMX00066

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using RMX00066

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (RMX00066) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RBM25 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00066

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-12 cells, Jurkat cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00066 targets RBM25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: RNA binding motif protein 25
• Calculated Molecular Weight: 843 aa, 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC113389
• Gene Symbol: RBM25
• Gene ID (NCBI): 58517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P49756
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.