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RBM7 Polyclonal antibody, PBS Only (21896-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM7 antibody (21896-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

RBM7 Polyclonal antibody, PBS Only

Name: RBM7 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21896-1-PBS

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21896-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21896-1-PBS targets RBM7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11106 Product name: Recombinant human RBM7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-266 aa of BC034381 Sequence: AAEADRTLFVGNLETKVTEELLFELFHQAGPVIKVKIPKDKDGKPKQFAFVNFKHEVSVPYAMNLLNGIKLYGRPIKIQFRSGSSHAPQDVSLSYPQHHVGNSSPTSTSPSRYERTMDNMTSSAQIIQRSFSSPENFQRQAVMNSALRQMSYGGKFGSSPLDQSGFSPSVQSHSHSFNQSSSSQWRQGTPSSQRKVRMNSYPYLADRHYSREQRYTDHGSDHHYRGKRDDFFYEDRNHDDWSHDYDNRRDSSRDGKWRSSRH Predict reactive species
Full Name	RNA binding motif protein 7
Calculated Molecular Weight	266 aa, 31 kDa
Observed Molecular Weight	30-35 kDa
GenBank Accession Number	BC034381
Gene Symbol	RBM7
Gene ID (NCBI)	10179
RRID	AB_2878938
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y580
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).