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  • KD/KO Validated

RBM8A,Y14 Polyclonal antibody

RBM8A,Y14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14958-1-AP

Synonyms

Binder of OVCA1 1, BOV 1, BOV 1A, BOV 1B, BOV 1C, MDS014, RBM8, RBM8A, RBM8A,Y14, RBM8B, Ribonucleoprotein RBM8A, RNA binding motif protein 8A, RNA binding protein 8A, RNA binding protein Y14, Y14, ZNRP, ZRNP1



Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human spleen tissue, human testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14958-1-AP targets RBM8A,Y14 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM8A,Y14 fusion protein Ag6800
Full Name RNA binding motif protein 8A
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC017088
Gene Symbol RBM8A
Gene ID (NCBI) 9939
RRIDAB_2178820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IHC protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IF protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IP protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseIF

RNA

An atlas of chromatoid body components.

Authors - Oliver Meikar
humanWB,IHC

Front Oncol

RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.

Authors - Yan Lin
  • KD Validated
humanWB

Cell Death Discov

CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.

Authors - Xiaojin Song