RBMS1 Polyclonal antibody

RBMS1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11061-2-AP

Synonyms

C2orf12, HCC 4, MSSP, MSSP 1, MSSP 2, MSSP 3, MSSP1, RBMS1, SCR2, YC1

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Validation Data Gallery

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  WB analysis using 11061-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11061-2-AP

  IP result of anti-RBMS1 (IP:11061-2-AP, 4ug; Detection:11061-2-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HepG2 using 11061-2-AP

  Immunofluorescent analysis of HepG2 cells, using RBMS1 antibody 11061-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, C6 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11061-2-AP targets RBMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBMS1 fusion protein Ag1493
• Full Name: RNA binding motif, single stranded interacting protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC018951
• Gene Symbol: RBMS1
• Gene ID (NCBI): 5937
• RRID: AB_2179121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Epigenetics; Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma; Authors - Hang Zhai