Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, C6 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11061-2-AP targets RBMS1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1493 Product name: Recombinant human RBMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-406 aa of BC018951 Sequence: MGKVWKQQMYPQYATYYYPQYLQAKQSLVPAHPMAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLPPHTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELENMLKPFGQVISTRILRDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPPGVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRPWHREGEVRLAGMTLTYDPTTAAIQNGFYPSPYSIATNRMITQTSITPYIASPVSAYQVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLGSTGTYMPATSAMQGAYLPQYAHMQTTAVPVEEASGQQQVAVETSNDHSPYTFQPNK Predict reactive species |
| Full Name | RNA binding motif, single stranded interacting protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC018951 |
| Gene Symbol | RBMS1 |
| Gene ID (NCBI) | 5937 |
| RRID | AB_2179121 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBMS1 antibody 11061-2-AP | Download protocol |
| IP protocol for RBMS1 antibody 11061-2-AP | Download protocol |
| WB protocol for RBMS1 antibody 11061-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBMS1 Polyclonal antibody (Cat# 11061-2-AP) has 8 total citations published in journals including Cancer Letters, J Transl Med, Int J Biol Macromol, EMBO Mol Med, BMC Cancer, Cell Cycle, Epigenetics, and Mol Cell Biol. The associated research fields span breast cancer chemoresistance, multiple myeloma progression, renal fibrosis, cardiac hypertrophy, head and neck squamous cell carcinoma, hepatocellular carcinoma ferroptosis, and cellular senescence.
| Species | Application | Title |
|---|---|---|
Cancer Lett tRF-1432 orchestrates RBMS1-IMPDH2 regulatory control to drive purine-dependent chemoresistance in breast cancer.
| ||
J Transl Med m6A methylation-modified ETV1 drives multiple myeloma progression and M2 polarization of tumor-associated macrophage through transcriptional activation of RBMS1. | ||
Int J Biol Macromol RNA binding motif single-stranded interacting protein 1 drives renal fibrosis by stabilizing Annexin A8 mRNA to activate PI3K/AKT signaling.
| ||
EMBO Mol Med RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics.
| ||
BMC Cancer RBMS1-HSPA8 axis activation drives head and neck squamous cell carcinoma progression | ||
Cell Cycle RBMS1 enhances PDPK1 mRNA stability to promote multiple myeloma malignancy and M2 macrophage polarization.
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