RBMS2 Monoclonal antibody, PBS Only

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• RBMS2 Monoclonal antibody, PBS Only (67395-1-PBS)

RBMS2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67395-1-PBS

Clone No.1E5C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

1E5C5, RNA-binding motif, single-stranded-interacting protein 2, SCR3, Suppressor of CDC2 with RNA-binding motif 3

• 

  WB analysis using 67395-1-Ig (same clone as 67395-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of LNCaP using 67395-1-Ig (same clone as 67395-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U2OS using 67395-1-Ig (same clone as 67395-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 67395-1-Ig (same clone as 67395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse adrenal gland using 67395-1-Ig (same clone as 67395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 67395-1-Ig (same clone as 67395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67395-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

67395-1-PBS targets RBMS2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27721

  Product name: Recombinant human RBMS2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-407 aa of BC027863

  Sequence: MLLSVTSRPGISTFGYNRNNKKPYVSLAQQMAPPSPSNSTPNSSSGSNGNDQLSKTNLYIRGLQPGTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPSAAQKAVTALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELEGMLKPFGQVISTRILRDTSGTSRGVGFARMESTEKCEAIITHFNGKYIKTPPGVPAPSDPLLCKFADGGPKKRQNQGKFVQNGRAWPRNADMGVMALTYDPTTALQNGFYPAPYNITPNRMLAQSALSPYLSSPVSSYQRVTQTSPLQVPNPSWMHHHSYLMQPSGSVLTPGMDHPISLQPASMMGPLTQQLGHLSLSSTGTYMPTAAAMQGAYISQYTPVPSSSVSVEESSGQQNQVAVDAPSEHGVYSFQFNK

• Full Name: RNA binding motif, single stranded interacting protein 2
• Calculated Molecular Weight: 407 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC027863
• Gene Symbol: RBMS2
• Gene ID (NCBI): 5939
• RRID: AB_2882639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15434
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBMS2 (RNA-binding motif, single-stranded-interacting protein 2), also known as SCR3 (Suppressor of CDC2 with RNA-binding motif 3), is a member of c-Myc single-strand binding proteins (MSSPs) family and encodes RNA binding protein. RBMS2 plays a critical role in the regulation of transcription and alternative splicing, both key cellular processes related to cell proliferation and programmed cell death or apoptosis (PMID: 40854446). Overexpression of RBMS2 inhibited CRC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) (PMID: 30514345, 38058408).