RBP3 Polyclonal antibody

RBP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14352-1-AP

Synonyms

D10S64, D10S65, D10S66, IRBP, RBP3, RBPI, Retinol binding protein 3

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Validation Data Gallery

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  WB analysis of mouse eye using 14352-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14352-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14352-1-AP

  IP result of anti-RBP3 (IP:14352-1-AP, 4ug; Detection:14352-1-AP 1:400) with Y79 cells lysate 2000ug.

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  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse eye tissue, Y79 cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14352-1-AP targets RBP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBP3 fusion protein Ag5699
• Full Name: retinol binding protein 3, interstitial
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC039844
• Gene Symbol: RBP3
• Gene ID (NCBI): 5949
• RRID: AB_2096956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)

Publications

Species	Application	Title
mouse	IP	J Exp Med; Identification of a novel cis-regulatory element essential for immune tolerance.; Authors - Taylor N LaFlam
human	IHC	Acta Biomater; Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.; Authors - Majed Felemban
human	WB	Proc Natl Acad Sci U S A; Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy.; Authors - Irene Tom
mouse	WB	Diabetes; Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-induced Retinal Dysfunction and Neurodegeneration through Rhodopsin.; Authors - Jianglei Chen
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	WB	Sci Rep; Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation; Authors - Mingzhu Yang