RCL1 Polyclonal antibody

• 

  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15330-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15330-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 15330-1-AP

  Immunofluorescent analysis of A375 cells using 15330-1-AP (RCL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RCL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15330-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RTC2, RPC2, RNAC, RNA 3'-terminal phosphate cyclase-like protein, HSPC338

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, A375 cells, MCF-7 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

15330-1-AP targets RCL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7058

  Product name: Recombinant human RCL1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-373 aa of BC001025

  Sequence: MATQAHSLSYAGCNFLRQRLVLSTLSGRPVKIRKIRARDDNPGLRDFEASFIRLLDKITNGSRIEINQTGTTLYYQPGLLYGGSVEHDCSVLRGIGYYLESLLCLAPFMKHPLKIVLRGVTNDQVDPSVDVLKATALPLLKQFGIDGESFELKIVRRGMPPGGGGEVVFSCPVRKVLKPIQLTDPGKIKRIRGMAYSVRVSPQMANRIVDSARSILNKFIPDIYIYTDHMKGVNSGKSPGFGLSLVAETTSGTFLSAELASNPQGQGAAVLPEDLGRNCARLLLEEIYRGGCVDSTNQSLALLLMTLGQQDVSKVLLGPLSPYTIEFLRHLKSFFQIMFKIETKPCGEELKGGDKVLMTCVGIGFSNLSKTLK

• Full Name: RNA terminal phosphate cyclase-like 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC001025
• Gene Symbol: RCL1
• Gene ID (NCBI): 10171
• RRID: AB_2180010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2P8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	J Mol Cell Biol; Stability and function of RCL1 are dependent on interaction with BMS1; Authors - Yong Wang
human	WB,IF	Cancer Cell Int; Rcl1 suppresses tumor progression of hepatocellular carcinoma: a comprehensive analysis of bioinformatics and in vitro experiments.; Authors - Yu Jiaze